Vol. 1 No. 1 (2023): Dynamic Science and Engineering Infrastructure-base for Achieving Innovations

FTIR characterization, phytochemical, antibacterial, and antioxidant properties of Pterocarpus Osun stembark and leaf extracts

Adewale Fadeyi
Sheda Science and Technology Complex, Abuja
Oluremi Isola Adeniran
Chemistry Department, University of Abuja, Nigeria
Abayomi Orishadipe
Chemistry Advanced Research Centre, Sheda Science and Technology Complex(SHESTCO), FCT, Abuja
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Published 2023-10-11


  • Secondary metabolites,
  • Pterocarpus osun,
  • antimicrobial,
  • DPPH,
  • Fabaceae

How to Cite

Fadeyi, A., Adeniran, O. I., & Orishadipe, A. (2023). FTIR characterization, phytochemical, antibacterial, and antioxidant properties of Pterocarpus Osun stembark and leaf extracts. Nigerian Journal of Science and Engineering Infrastructure, 1(1). Retrieved from https://njsei.naseni.gov.ng/index.php/nsjei/article/view/45


Antimicrobial resistance threatens the prevention and treatment of an ever-increasing range of infections caused by microorganisms. It becomes necessary therefore, to determine compounds from natural sources that can be used to develop novel medicines with potent antimicrobial properties. Although, the stem bark of Pterocarpus osun has long been used to treat diarrhea, dysentery, and other gastrointestinal problems, there are no published pharmacological reports to authenticate these uses. The leaf and stem bark of Pterocarpus osun were extracted exhaustively and successively with n-hexane, ethyl acetate and methanol. The antioxidant capacities of the extracts were measured using the 2,2-Diphenyl-1-PicrylHydrazyl (DPPH) scavenging technique and IC50 calculated. The extracts were screened for secondary metabolites and antibacterial activities. The result of the DPPH scavenging activities of the crude fractions compared favorably with that of the ascorbic acid used as standard control. 85.0% activity was recorded at concentration of 62.5µg/mL for the methanol stem bark fraction and, the least activity of 62.7% for ethyl acetate leaf extract at a concentration of 1000µg/mL. Terpenes, saponins, steroids, alkaloids, tannins, and flavonoids were detected in the extracts. Four of the eleven human pathogens used for antimicrobial screening were sensitive, four others were resistant. Zones of inhibition measured ranged between 18 and 29mm. Minimum inhibitory concentration and minimal bacteria/fungicidal concentrations were measured between 1.25 to 5.00mg/mL. The FTIR spectra of the powdered samples and extracts’ fractions reported.